A strong CoA makes verification straightforward. Here’s how to interpret the common sections and spot the details that matter.
Identity Confirmation
- HPLC retention time: Should match the reference profile.
- Mass spectrometry (MS): Expected molecular weight peak present.
- Sequence confirmation: When provided, confirms order and modifications.
Purity Reporting
- % Purity (HPLC): Typically reported as area normalization; note method specifics.
- Impurity profile: Minor peaks listed where applicable; look for thresholds and identification notes.
Lot and Labeling
- Lot/Batch number: Enables traceability across production and fulfillment.
- Manufacture/analysis dates: Useful for shelf‑life planning.
Methods and Conditions
- HPLC method: Column type, gradient, wavelength (e.g., 214 nm).
- MS method: Ionization source (e.g., ESI), instrument model.
Acceptance Criteria
- Ensure the measured values meet your lab’s specification limits.
Documentation Hygiene
- Save PDFs in your LIMS/drive by product + lot.
- Cross‑reference with inventory labels and SDS.
RUO Notice: CoAs support research traceability; not a clinical validation report.
